Interleukin 6 Induces the Expression of Vascular Endothelial Growth Factor

We demonstrate using Northern analysis that treatment of various cell lines with IL-6 for 6 – 48 h results in a significant induction of Vascular endothelial growth factor mRNA

Tzafra Cohen

2002

Scholarcy highlights

  • Angiogenesis, the formation of new blood vessels, is a multistep process in which many growth factors and cytokines have an essential role
  • The results shown in Fig. 2 clearly demonstrate that Vascular endothelial growth factor mRNA expression in the two cell lines was augmented by both IL-6 and cobalt
  • We provide evidence indicating that IL-6 may promote angiogenesis through the induction of VEGF expression
  • This induced expression is mediated by specific DNA motifs located on the putative promoter region of VEGF as well as by specific elements located in the 5Ј-UTR
  • The first is located between positions Ϫ796 and Ϫ804 within the 5Ј-UTR and the second is located between positions Ϫ2313 and Ϫ2320 upstream to the transcription initiation site
  • Internal deletions within the 5Ј-UTR abrogated the effect of IL-6, indicating that a possible functional IL-6-RE is contained within that region. These findings indicate that the putative IL-6-REs at the promoter region of VEGF and its 5Ј-UTR are functional and operate in a cooperative manner
  • A deletion of 83 bp located in the vicinity of the translation start site resulted in a significant decrease in chloramphenicol acetyltransferase activity, suggesting that this region has a role in Vascular endothelial growth factor expression

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