Nuclear Import of the Yeast AP-1-like Transcription Factor Yap1p Is Mediated by Transport Receptor Pse1p, and This Import Step Is Not Affected by Oxidative Stress

We examined the mechanism for Yap1p nuclear import, and determined whether the import step is affected by oxidative stress

Takeshi Isoyama; Asako Murayama; Akio Nomoto; Shusuke Kuge

2002

Scholarcy highlights

  • Regulation of the nucleocytoplasmic transport of transcription factors is, in most cases, a crucial step for the transmission of extracellular signals such as stress and growth stimuli to the nucleus
  • We examined the mechanism for Yap1p nuclear import, and determined whether the import step is affected by oxidative stress
  • We identified the nuclear localization signal of Yap1p and demonstrated that the nuclear import of Yap1p was not affected by oxidative stress
  • This nuclear accumulation was inhibited at the restrictive temperature, indicating clearly that the nuclear import of Yap1p is mediated in a Ran-dependent manner
  • We examined the molecular mechanism of the import step of Yap1p and determined whether the import step of Yap1p was affected by oxidative stress
  • The nuclear transport of Yap1p was strongly prevented in pse cells at the restrictive temperature, whereas none of the other mutant cells carrying each one of the genes encoding an importin ␤ family member as well as an adaptor molecule that we tested affected the nuclear import of Yap1p
  • Our results and previous studies clearly indicate that Yap1p subcellular localization in response to oxidative stress is regulated solely at the step of Crm1p-dependent nuclear export

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