The N gene of tobacco confers resistance to tobacco mosaic virus in transgenic tomato.

We demonstrate that N confers resistance to tobacco mosaic virus in transgenic tomato

S. Whitham; S. McCormick; B. Baker

2002

Scholarcy highlights

  • It has been proposed that cloned plant disease resistance genes could be transferred from resistant to susceptible plant species to control important crop plant diseases
  • In contrast to the transgenic tomato plants, tobacco mosaic virus-induced hypersensitive response lesions were observed at -48 hr postinoculation on Samsun NN, which is typical for tobacco
  • The major finding of this study is that we have demonstrated that a member of the nucleotide binding site/leucine-rich region class of disease resistance genes can be transformed into another crop plant species, where it confers resistance to a significant pathogen
  • The N gene mediates resistance to the viral pathogen TMV by the formation HR lesions and inhibition of TMV movement in transgenic tomato, as it does in tobacco
  • The finding that N-mediated TMV resistance is reconstituted in transgenic tomato demonstrates that all of the components required by N for both TMV recognition and signal transduction are conserved in tomato
  • N confers resistance to TMV in a genetically tractable species where genetic tools and a powerful selection for loss-of-function mutations using the temperature shift protocol can be used to identify genes encoding the components of the signal transduction pathway leading to HR and resistance to TMV
  • It will be interesting to test if N-mediated resistance to tobacco mosaic virus can reconstituted in plant species more distantly related to tobacco, such as the crucifers, which can be hosts for tobamovirus infection

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