Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.

The applicability of small subunit ribosomal RNA sequences for bacterial dassification is well accepted, the general use of these molecules has been hindered by the technical difficulty of obtaining their sequences

D. J. Lane

2006

Scholarcy highlights

  • The applicability of small subunit ribosomal RNA sequences for bacterial dassification is well accepted, the general use of these molecules has been hindered by the technical difficulty of obtaining their sequences
  • Of the macromolecules used for phylogenetic analysis, the ribosomal RNAs, 16S rRNA,l have proven the most useful for establishing distant relationships because of their high information content, conservative nature, and universal distribution
  • Using RNase T1 oligonucleotide catalogs of 16S rRNA, Woese and his colleagues were able to establish a comprehensive outline of prokaryotic phylogeny
  • The principle of using rRNA sequences to characterize microorganisms has gained wide acceptance, and its general application can be anticipated if methods for determining rRNA sequences can be simplified
  • What is important is that the number of nucleotides compared is statistically meaningful and that com
  • In order to assess inaccuracies in data gathered by using reverse transcriptase, the results of elongation from the described primers were inspected by using rRNA templates for which the gene sequences were known
  • The novel aspect is the use of universally applicable, oligodeoxynucleotide primers to access 16S rRNA sequences for phylogenetic characterizations

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