Expression of cellular homologues of retroviral onc genes in human hematopoietic cells.

Our present study shows that the human homologues of the onc genes of avian myelocytomatosis virus strain MC29, Abelson. murine leukemia virus, Harvey murine sarcoma virus, and simian sarcoma virus are expressed in some or all human hematopoietic cells tested

E. H. Westin

2006

Scholarcy highlights

  • Total cellular poly(A)-enriched RNA from.a variety of fresh human leukemic blood cells and hematopoietic cell lines was analyzed for homology with molecularly cloned DNA probes containing the one sequence of Abelson. murine leukemia virus, Harvey murine sarcoma virus, simian sarcoma virus, and avian myelocytomatosis virus strain MC29
  • A single 5.8-kilobase species of Ab-MuLV mRNA was detected with as little as 0.25,ug of total cellular RNA applied to the gel
  • Our present study shows that the human homologues of the onc genes of MC29, Ab-MuLV, Harvey murine sarcoma virus, and SSV are expressed in some or all human hematopoietic cells tested
  • A parallel study examining human solid tumor cell lines and normal fibroblasts with the same abl probe and the viral onc gene of BALB-MuSV, which is closely related to Harvey-ras, revealed widespread transcription of multiple species of mRNA
  • These results are consistent with the interpretation that these genes may have an important role in normal cellular functions
  • It has been shown that a mouse erythroid precursor cell line expressed high levels of a p21 protein assumed to be the product of c-Harvey-ras, suggesting a correspondence of the pathogeneity of v-Harvey-ras with the in vivo tissue specificity for expression of the cellular gene
  • Our results failed to show a correlation of high-level expression of the onc genes examined with specific types of leukemias

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