The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.

In Results we have presented data that locate the DNA-binding sites of the proteins involved in the joint regulation of the arabinose operon promoters PC and PBAD

S. Ogden

2006

Scholarcy highlights

  • The locations of DNA binding by the proteins involved with positive and negative regulation of transcription initiation of the L-arabinose operon in Escherichia coli have been determined by the DNase I protection method
  • The regulatory region DNA has been isolated, and its nucleotide sequence has been determined and found to contain elements similar to the RNA polymerase-binding sites seen in other E. coli promoters at about 10 and 35 bases before the start sites of transcription
  • In the other experiment shown, the original data showed more complete protection of CRPBAD than CRPC, the reproduced data show the differences less well. From these observations we conclude that the in vitro affinity of CRP protein is higher for CRPBAD than it is for CRPC
  • The partial overlapping of araO with both CRPBAD and CRPC suggested that the repression complex of araC protein with DNA might repress both the PBAD and PC promoters; induction by arabinose might both induce PBAD and derepress Pc
  • Several years ago Casadaban addressed this very question after noting that both ara promoters were in the same region
  • In Results we have presented data that locate the DNA-binding sites of the proteins involved in the joint regulation of the arabinose operon promoters PC and PBAD
  • We have described a mechanism of gene regulation in which induction occurs only when all of a set of proteins are present and able to bind to a string of sites on DNA

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