Tirofiban Blocks Platelet Adhesion to Fibrin with Minimal Perturbation of GpIIb/IIIa Structure

Our results provide new insights into the mechanisms and potential prothrombotic complications of integrin antagonists

Mary Stahle

2018

Scholarcy highlights

  • A biophysical approach tested the hypothesis that tirofiban, like eptifibatide, perturbs GpIIb/IIIa structure
  • Electrophoretic analyses demonstrated that tirofiban protected GpIIb/IIIa from SDSinduced subunit dissociation
  • Saturating tirofiban concentrations had little or no effect on GpIIb/IIIa secondary or tertiary structure, as determined by circular dichroic spectroscopy, dynamic light scattering, and sedimentation velocity measurements performed with purified receptors in octyl glucoside
  • Moderate dose-dependent effects on GpIIb/IIIa quaternary structure were detected by sedimentation equilibrium
  • Our results provide new insights into the mechanisms and potential prothrombotic complications of integrin antagonists

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