Crm1 (XpoI) dependent nuclear export of the budding yeast transcription factor yAP‐1 is sensitive to oxidative stress

We have shown previously that yAP-1 is mainly found in the cytoplasm, but that upon the imposition of oxidative stress it localizes to the nucleus

Shusuke Kuge; Takashi Toda; Narushi Iizuka; Akio Nomoto

2003

Scholarcy highlights

  • The yAP-1 transcription factor is crucial for the oxidative stress response of the budding yeast Saccharomyces cerevisiae; its activity is induced in response to oxidative stress, and as a consequence the expression of a number of target genes is enhanced
  • We addressed the mechanism through which yAP-1 nuclear localization is regulated
  • We show that yAP-1 localization is mediated by active export from the nucleus, resulting from the activity of Crm1, a conserved protein that functions as an export receptor which recognizes the nuclear export signal
  • When Crm1 expression was repressed, yAP-1 was localized in the nucleus and induced the expression of a yAP-1 dependent target gene
  • Our results suggest that the cysteine rich domain, at the C-terminus of yAP-1, functions as an export recognition sequence. yAP-1 and Crm1 interact in vivo and this interaction is reduced in response to oxidative stress
  • These results suggest a novel regulatory mechanism of nucleocytoplasmic transport which is dependent upon a redox sensitive nuclear export pathway

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