Neurotrophin-4 Production by Human Epidermal Keratinocytes: Increased Expression in Atopic Dermatitis

This study demonstrates differential expression and regulation of neurotrophins in human skin

Markus Grewe

2003

Scholarcy highlights

  • Chronic inammatory conditions of human skin, such as prurigo lesions of atopic dermatitis, are characterized clinically by intense pruritus and histologically by increased innervation
  • Expression of neurotrophin 1Botchkarev VA-4 mRNA was signi®cantly induced by treatment of human keratinocytes with IFN-g or phorbol 12-myrisate 13-acetate, while treatment with dexamethasone or, as diluent controls, with 0.01% bovine serum albumin and 0.1% ethanol failed to do so
  • We report that human epidermal keratinocytes express and secrete biologically active NT-4
  • This conclusion is based on demonstration of expression of mRNA speci®c for NT-4; the presence of a protein reacting speci®c to antihuman NT-4 antibody in immunoprecipitates of HNK culture supernatants and HNK lysates, and the presence of a glioblastoma-growth-promoting biological activity in HNK culture supernatants, which was neutralized by anti-NT-4 antibodies
  • In HNK cultures, keratinocyte NT-4 production was markedly induced by the proinammatory cytokine IFN-g
  • In support of this assumption we have observed that NT-4 is highly expressed in a chronic inammatory skin disease, i.e., atopic dermatitis
  • Epidermal neurotrophin 1Botchkarev VA-4 expression is markedly increased in IFN-g-injected skin as well as in atopic dermatitis lesions, whereas under both conditions NT-3 expression was nearly unaltered

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