Temporal and spatial expression of a thiolprotease gene during pea ovary senescence, and its regulation by gibberellin [published erratum appears in Plant J 1993 Apr;3(4):627]

In this paper we describe the isolation and characterization of a cDNA encoding a thiolprotease from pea

A Granell


Scholarcy highlights

  • Senescence represents the last stage in plant development
  • The sequence of the tpp cDNA displays a high similarity with other plant and animal thiolproteases of the papain group
  • The homology is highest around the Cys-His of the active centre; a 109 amino acid sequence at the carboxy terminus was foundto be homologousonlyto thiolproteasesof plant origin; this part of the mRNA is present in another pea mRNA that exhibits similar patterns of induction. tpp mRNA shows a temporal pattern of accumulation that precedes that observed for proteolytic activity
  • Such accumulation did not occur when ovaries were inducedto grow parthenocarpicallyby gibberellic acid treatment; the initial low level of expression present in ovaries decreased after GA treatment, indicating that the gene is down-regulated by gibberellins
  • To initiatea molecular examination of the proteaseactivity associated with pea ovary senescence, total and poly(A)+ RNA were extracted and analysed by Northernblots using as probe a heterologous cDNA for a cold-induced thiolprotease from tomato
  • A major feature of the senescing ovaries is the development of localized protease activity; such activity does not develop in ovaries induced to grow parthenocarpically by treatment with GA.The results in this paper have described the cloning, and sequence of a cDNA of a thiolprotease which shows temporal and spatial patterns of induction closely related to the patterns of protease activity which develop during ovary senescence
  • This pattern of expression precedes the development of the cytopathogenic effects observed as unpollinated ovaries undergo senescence

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