A biologically active IL-1beta derived peptide stimulates phagocytosis and bactericidal activity in rainbow trout, Oncorhynchus mykiss (Walbaum), head kidney leucocytes in vitro

The present work provides the first information concerning the ability of a rainbow trout interleukin-1β derived peptide, P3, to enhance phagocytosis and bactericidal activity

S Peddie; J Zou; C J Secombes

2003

Scholarcy highlights

  • The present work provides the first information concerning the ability of a rainbow trout interleukin-1β derived peptide, P3, to enhance phagocytosis and bactericidal activity
  • P3 was synthesized to complex with the IL-1 receptor and corresponds to fragment 197–206 of the trout sequence
  • With respect to the temporal kinetics of P3 induced activation, an incubation period of 2 h resulted in enhanced phagocytic activity, incubation times of between 4 and 24 h were optimal
  • With respect to bactericidal activity, macrophages incubated for 24 h with 0.1 mM P3 demonstrated an enhanced ability to kill an avirulent strain of the Gram-negative salmonid pathogen Aeromonas salmonicida
  • Further investigation revealed that extracellular production of superoxide anions was not responsible for the enhanced bactericidal activity of macrophages exposed to P3

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