Regulation of reactive-oxygen-species generation in fibroblasts by Rac 1

We demonstrate that in NIH 3T3 cells transient expression of constitutively activated forms of the small GTP-binding proteins Ras or Rac1 leads to a significant increase in intracellular reactive oxygen species

Maitrayee SUNDARESAN; Zu-Xi YU; Victor J FERRANS; David J SULCINER; Silvio GUTKIND; Kaikobad J. IRANI; Pascal J. GOLDSCHMIDT-CLERMONT; Toren FINKEL

2015

Scholarcy highlights

  • Reactive oxygen species are conventionally viewed as toxic by-products of cellular metabolism, a growing body of evidence suggests that they may regulate signal transduction in both plant and animal cells
  • To measure intracellular reactive oxygen species concentration, NIH 3T3 cells were incubated with the fluorophore dichlorofluorescin diacetate
  • The family of Ras-related small GTP-binding proteins is thought to play a pivotal role in multiple signal-transduction pathways
  • Given that the generation of ROS has been shown to play an essential role in growth factor and cytokine stimulation, we thought that it was possible that Rac or related proteins may regulate ROS in non-phagocytic cells
  • In the present paper we demonstrate that expression of constitutively active mutants of Ras or Rac1 leads to a rise in intracellular ROS in fibroblasts
  • In NIH 3T3 cells the increase in ROS following growth factor and cytokine stimulation requires Rac1
  • In the present paper we demonstrate that expression of constitutively active mutants of Ras or Rac1 leads to a rise in intracellular reactive oxygen species in fibroblasts

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