An aluminium-based fluorinated counterion for enhanced encapsulation and emission of dyes in biodegradable polymer nanoparticles

To minimize aggregation-caused quenching of the loaded dyes, we have recently proposed the use of cationic dyes with bulky hydrophobic counterions, which serve as spacers preventing dye pi-stacking inside nanoparticles

Bohdan Andreiuk; Andreas Reisch; Vasyl G. Pivovarenko; Andrey S. Klymchenko

2017

Scholarcy highlights

  • Research ArticleMaterials Chemistry Frontiers minimal leaching inside cells, relatively high selfquenching was observed at high dye loading. The use of bulky side groups can significantly decrease self-quenching, as exemplified in the case of BODIPY16 and perylenediimide derivatives
  • We showed that R18/F5-TPB inside PLGA NPs displayed very low anisotropy even for relatively low dye loading, which suggested that F5-TPB brings dyes in close proximity within the PLGA matrix and ensures fast energy transfer
  • We introduced a concept of counterion-enhanced emission of dyes inside polymer nanoparticles using perfluorinated tetraphenylborate, which enabled preparation of ultrabright switchable fluorescent NPs
  • Our results show that F9-Al can perfectly replace F5-TPB for dye encapsulation, because it effectively prevents aggregation-caused quenching, ensures high brightness of NPs and favours collective behaviour of encapsulated dyes
  • F9-Al enhances encapsulation of rhodamine dyes into PLGA NPs, as they did not show any sign of leakage after entering living cells, in contrast to NPs loaded with the dye perchlorate salt
  • Sodium phosphate monobasic and sodium phosphate dibasic dihydrate were used to prepare 20 mM phosphate buffer solutions at pH 7.4
  • Cellular imaging data suggested that F9-Al encapsulates cationic rhodamine even more efficiently than F5-TPB, which can be assigned to the higher hydrophobicity of F9-Al compared to F5-TPB

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