RNAi-mediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivo

We show that the noncovalent complexation of synthetic small interfering RNAs with low molecular weight polyethylenimine efficiently stabilizes siRNAs and delivers siRNAs into cells where they display full bioactivity at completely nontoxic concentrations

B Urban-Klein; S Werth; S Abuharbeid; F Czubayko; A Aigner

2004

Scholarcy highlights

  • RNA interference represents a powerful, naturally occurring biological strategy for inhibition of gene expression
  • The application of small interfering RNAs is severely limited by the instability and poor delivery of unmodified siRNA molecules into the cells in vivo
  • We show that the noncovalent complexation of synthetic siRNAs with low molecular weight polyethylenimine efficiently stabilizes siRNAs and delivers siRNAs into cells where they display full bioactivity at completely nontoxic concentrations
  • We establish a novel and simple system for the systemic in vivo application of small interfering RNAs through PEI complexation as a powerful tool for future therapeutic use

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