Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4

We describe the role of reactive oxygen species as signaling molecules in starvation-induced autophagy

Ruth Scherz-Shouval; Elena Shvets; Ephraim Fass; Hagai Shorer; Lidor Gil; Zvulun Elazar

2007

Scholarcy highlights

  • Autophagy is a major pathway for delivery of proteins and organelles to lysosomes/the vacuole, where they are degraded and recycled
  • To rule out the possibility that the oxidative signal associated with starvation is leading to programmed cell death, we tested the viability of cells grown under nutrient starvation conditions
  • Taken together with the finding that the oxidative signal appears minutes after induction of starvation, these results suggest that H2O2 serves as a signaling molecule in the autophagic pathway, and not as part of an oxidative stress leading to PCD
  • Many of the proteins involved in this process have been identified, the signal transduction pathway leading to activation of autophagy is not fully solved
  • We show that starvation triggers accumulation of reactive oxygen species, most probably H2O2, which is necessary for autophagosome formation and the resulting pathway of degradation
  • We found nearly 40% less of the lipidated form of GATE-16 in cells transfected with HsAtg4AC81S as compared with cells transfected with HsAtg4AWT
  • We identify a direct target for oxidation by H2O2—the protease Atg4
  • Redox regulation of Atg4 can provide rapid activation and inactivation of this protease, as part of the signaling pathway, leading to induction of the autophagic process

Need more features? Save interactive summary cards to your Scholarcy Library.