Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion

We found that amoeboid fibrosarcoma cells’ membrane blebbing is enhanced upon constriction and is occasionally present in mesenchymally invading cells around constricted nuclei

Ondřej Tolde

2018

Scholarcy highlights

  • Cancer cell invasion is the crucial step in the process of metastasis formation, which is responsible for 90% of deaths in patients with solid tumours
  • We take advantage of coherence - controlled holographic microscopy to visualize in detail dynamics of cancer cell invasive behaviour, with focus on the interaction of cancer cells with collagen fibres, and utilize the quantitative information contained in the acquired images for analysis of cellular mass distribution and translocation
  • Their migration in 3D collagen is unaffected in the presence of GM6001, a broad-spectrum matrix metalloproteinase inhibitor, unlike migration of control mesenchymal cells that is stalled in such conditions (Suppl
  • Detailed visualization of live cells in 3D collagen as reported here was enabled by coherence gate effect, which is characteristic of quantitative phase imaging in the spatially incoherent light
  • Taking advantage of the CCHM we investigated in detail the features of cancer cells utilizing either the amoeboid or mesenchymal invasion mode and analysed cell mass distribution changes within the migrating cancer cell
  • An interesting observation visualized by CCHM was bundling of several collagen fibres together by pseudopodia-like protrusions of a mesenchymal cell
  • Observations presented here using coherence - controlled holographic microscopy demonstrate that cancer cells can temporally and dynamically utilize characteristics of both the amoeboid and mesenchymal phenotype and adjust their modes of invasion according to current conditions

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