Root-derived GA12 contributes to temperature-induced shoot growth in Arabidopsis

We report that root-derived GA12, a precursor of the bioactive gibberellins, mediates thermo-responsive shoot growth in Arabidopsis

Lucie Camut; Thomas Regnault; Mathilde Sirlin-Josserand; Lali Sakvarelidze-Achard; Esther Carrera; Julie Zumsteg; Dimitri Heintz; Nathalie Leonhardt; Maria João Pimenta Lange; Theo Lange; Jean-Michel Davière; Patrick Achard

2019

Scholarcy highlights

  • To cite this version: Lucie Camut, Thomas Regnault, Mathilde Sirlin-Josserand, Lali Sakvarelidze-Achard, Esther Carrera, et al
  • Root-derived GA12 contributes to temperature-induced shoot growth in Arabidopsis Lucie Camut, Thomas Regnault, Mathilde Sirlin-Josserand, Lali Sakvarelidze-Achard, Esther Carrera, Julie Zumsteg, Dimitri Heintz, Nathalie Leonhardt, Maria João Pimenta Lange, Theo Lange, Jean-Michel Davière and Patrick Achard1*
  • We found that ga20ox triple mutant rootstocks, which produce GA12 but are unable to convert it into GA15 and following products, were able to increase the growth of ga grafted scions at high temperature; consistent with the premise that GA12 is a mobile growth signal in Arabidopsis
  • We found that elevated temperature decreased the abundance of the DELLA protein REPRESSOR OF GA1-3 in hypocotyls of wild-type, kao kao pARSK1:KAO1-RFP and pif101 mutant but not in ga20ox pARSK1:GA20ox1-RFP hypocotyls; confirming that GA signaling acts upstream of PIF4
  • Consistent with the above results, we found that INDOLE-3-ACETIC ACID INDUCIBLE 19, IAA29, YUCCA 8 and PACLOBUTRAZOL RESISTANT 1 transcripts were increased by about twofold in wildtype and kao kao pARSK1:KAO1-RFP hypocotyls treated at 28°C, compared with those kept at 20°C
  • We demonstrated that root-derived GA12 contributes substantially to temperature-induced shoot growth, consistent with previous data showing that GA12 is the main mobile GA signal over long distances in Arabidopsis
  • GA12 content in xylem sap collected from ga scions of Col-0/ga grafted plants was determined by ultra-performance liquid chromatography-tandem mass spectrometry as described previously

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