SLR1 inhibits MOC1 degradation to coordinate tiller number and plant height in rice

We show that rice tiller number regulator MONOCULM 1 is protected from degradation by binding to the DELLA protein SLENDER RICE 1

Zhigang Liao; Hong Yu; Jingbo Duan; Kun Yuan; Chaoji Yu; Xiangbing Meng; Liquan Kou; Mingjiang Chen; Yanhui Jing; Guifu Liu; Steven M. Smith; Jiayang Li

2019

Scholarcy highlights

  • The breeding of cereals with altered gibberellin signaling propelled the ‘Green Revolution’ by generating semidwarf plants with increased tiller number
  • Among the genes involved in determining tiller number in rice, MONOCULM 1 is one of the most important because it is required for the formation of axillary meristems and subsequent bud outgrowth
  • We found that GAdeficient or GA-signaling mutants, including semidwarf 1, a GA biosynthesis mutant defective in GA20ox-2, sdg, and slr1-d1, a GA-signaling mutant, produced more tillers and had a shorter stature than the WT plants
  • We propose a model to illustrate how GA signaling via SLENDER RICE 1 directly coordinates plant height and tiller number
  • We present new evidences that GA signaling controls tiller number by directly controlling the level of MOC1 protein, which is protected from degradation through the interaction with the DELLA protein SLR1
  • GA signaling acting through SLR1 can promote shoot elongation independently of MOC1, as shown in the GID1 null mutant by CRISPR/Cas9 moc1CR double mutant
  • For cell-free protein degradation assays, full-length MONOCULM 1 was amplified using primers and cloned into the pMAL-c2x vector

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