MicroRNA-21 promotes cell transformation by targeting the programmed cell death 4 gene

MicroRNAs are small noncoding RNA molecules that negatively control expression of target genes in animals and plants

Z Lu; M Liu; V Stribinskis; C M Klinge; K S Ramos; N H Colburn; Y Li

2008

Scholarcy highlights

  • MicroRNAs are small noncoding RNA molecules that negatively control expression of target genes in animals and plants
  • Deletion of the putative miR-21-binding site from the 3′-UTR of programmed cell death 4, or mutations in the MRE abolished the ability of miR-21 to inhibit reporter activity, indicating that this MRE is a critical regulatory region
  • Western blotting showed that Pdcd4 protein levels were reduced by miR-21 in human and mouse cells, whereas quantitative real-time PCR revealed little difference at the mRNA level, suggesting translational regulation
  • The demonstration that miR-21 promotes cell transformation supports the concept that mir-21 functions as an oncogene by a mechanism that involves translational repression of the tumor suppressor Pdcd4

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