Long-term multiple color imaging of live cells using quantum dot bioconjugates

We have developed procedures for using quantum dots to label live cells and have demonstrated their use for long-term multicolor imaging of live cells

Jyoti K. Jaiswal; Hedi Mattoussi; J. Matthew Mauro; Sanford M. Simon

2003

Scholarcy highlights

  • Luminescent quantum dots—semiconductor nanocrystals—are a promising alternative to organic dyes for fluorescence-based applications
  • We have developed procedures for using QDs to label live cells and have demonstrated their use for long-term multicolor imaging of live cells
  • The two approaches presented are endocytic uptake of QDs and selective labeling of cell surface proteins with QDs conjugated to antibodies
  • Live cells labeled using these approaches were used for long-term multicolor imaging
  • The cells remained stably labeled for over a week as they grew and developed. These approaches should permit the simultaneous study of multiple cells over long periods of time as they proceed through growth and development

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