The Antimicrobial Protein Psoriasin (S100A7) Is Upregulated in Atopic Dermatitis and after Experimental Skin Barrier Disruption

As E. coli infections are not observed in atopic skin we investigated the functional role of psoriasin in atopic dermatitis patients

Regine Gläser

2008

Scholarcy highlights

  • The epidermis is constantly exposed to a variety of microbial challenges
  • Psoriasin immunoreactivity and secretion is enhanced in atopic dermatitis skin in vivo The expression of psoriasin in the epidermis was first examined at the protein level by immunostaining using a specific antibody
  • To investigate whether the increased psoriasin expression in the skin of patients with atopic dermatitis leads to enhanced secretion of psoriasin, washing fluids of standardized areas of nonlesional and lesional skin were obtained and analyzed for psoriasin concentrations using a specific psoriasin ELISA
  • A markedly enhanced secretion with up to 1500-fold increase of soluble psoriasin was observed in the washing fluids derived from AD lesional skin when compared to nonlesional skin and normal controls
  • The identification of various antimicrobial proteins and -3, psoriasin, and calprotectin) from lesional psoriatic skin might explain the phenomenon that these patients suffer from significantly fewer skin infections than expected
  • Collection of skin-derived washing fluids To determine the active secretion of psoriasin, standardized areas of untreated lesional and nonlesional skin of AD patients were rinsed with 900 ml of 100 ml sodium-phosphate buffer sodium-phosphate buffer and a corresponding localization was rinsed in the normal controls
  • Experimental disruption of the skin barrier Tape stripping of a standardized area of the forearm skin was performed in healthy volunteers until a transepidermal water loss of 40 g/m2 per hour monitored by the Tewameter TM210 was reached

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