Correction: Light-emitting diodes based on conjugated polymers

We found no significant dependence of the speed of bead movement on the kinesin density; average speeds all fell in the range 0.35-0.45 μm s- 1 The rate-limiting step for movement seems not to be associated with the reassociation step following release

J. H. Burroughes; D. D. C. Bradley; A. R. Brown; R. N. Marks; K. Mackay; R. H. Friend; P. L. Burn; A. B. Holmes


Scholarcy highlights

  • LETTERS TO NATURE from tubulin and diffuse for -1 μs, yet still have a 99% chance of returning to the substrate within 10 ms, a time sufficiently short for movement to appear largely unaffected
  • The occcasionally jittery character of bead motion driven by low numbers of kinesin molecules, including events of the kind shown in Fig. le-J, might be explained by a small number of longer-lived detached states
  • It has not been possible to relate this motion to the action of single molecules
  • The spontaneous bead detachments that we observe might be due to kinesin molecules operating with just a single active head, if, for example, the second head were inactivated by being bound to the bead surface
  • Diffusional rotation of a moving microtubule about its long axis will present an equivalent surface lattice of potential binding sites, whereas rotation of a bead carrying a few kinesin molecules can destroy the possibility of favourable binding
  • The existence of a detached state calls into question the feasibility of using tension probes, such as optical tweezers or glass microneedles, to measure the force of translocation for beads or microtubules that are driven by small numbers of motors
  • Measurements of the average force generated by larger numbers of kinesin molecules may be more meaningful

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