Exponential Amplification Using Photoredox Autocatalysis

We report a new exponential amplification strategy based on photoredox autocatalysis, where eosin Y, a photocatalyst, amplifies itself by activating a non-fluorescent eosin Y derivative under green light

Seunghyeon Kim; Alejandra Martínez Dibildox; Alan Aguirre-Soto; Hadley D. Sikes

2021

Scholarcy highlights

  • The development of rapid, affordable, and highly sensitive biodetection methods for resource-limited settings is of paramount importance in detection and diagnosis of infectious diseases
  • Synthesis and characterization of EYH2 Synthesis of EYH2 was achieved by reducing 1,4-benzoquinone-like structure of eosin Y to hydroquinone-like structure with sodium borohydride in aqueous solution as described in Figure 1A,42 leading to a loss of conjugation in the π-system
  • It should be noted that EY and EYH2 represent eosin Y and dihydroeosin Y in this paper, regardless of actual charges of the molecules
  • DAB polymerization was combined with EY amplification to enable visual detection of EY
  • Using EYconjugated affinity reagents, we developed a fluorescence assay for detection of SARS-CoV-2 nucleocapsid protein, a biomarker of COVID-19, and a colorimeric assay for detection of streptavidin, revealing that the EY amplification strategy can be adopted to amplify biodetection signals in different kinds of assays
  • Considering the long shelflife of EYH2 and the rapid EY amplification under low intensity light, we anticipate that this non-enzymatic autocatalytic amplification reaction is especially useful in developing rapid and highly sensitive point-of-care assays

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