Simultaneously Detecting Monoamine Oxidase A and B in Disease Cell/Tissue Samples Using Paper-Based Devices

As enzymes in the outer membrane of the mitochondrion, monoamine oxidases can catalyze the oxidative deamination of monoamines in the human body

Haixiao Fang; Meirong Wu; Wenhui Ji; Limin Wang; Yipei Chen; Ding Chen; Naidi Yang; Qiong Wu; Changmin Yu; Jie Liu; Jinhua Liu; Hua Bai; Bo Peng; Xiao Huang; Hai-Dong Yu; Lin Li

2020

Scholarcy highlights

  • As enzymes in the outer membrane of the mitochondrion, monoamine oxidases can catalyze the oxidative deamination of monoamines in the human body
  • The imbalance of the MAO-A is associated with neurological degeneration, while excess MAO-B activity is closely connected with Parkinson’s disease and Alzheimer’s disease; detection of MAOs is of great significance for the diagnosis and treatment of these diseases
  • This work reports the multiplexed detection of MAO-A and MAO-B using paper-based devices based on chemiluminescence
  • While only one antibody can be incubated on the same membrane by Western blotting, multiple antibodies incubated on the same paper enabled simultaneous detection of MAO-A and MAO-B by paper-based devices
  • The paper-based assay could be used for preliminary early screening of clinical samples for MAOs and can be extended as an alternative to WB for multiplexed detection of various proteins in disease cell or tissue samples
  • Such files may be downloaded by article for research use

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