Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues

We present here a practical and robust method, which we call DNAExchange-Imaging, for rapid in situ spectrally-unlimited multiplexing

Yu Wang


Scholarcy highlights

  • We show that the DNA-Exchange-Imaging principle can be generalized to other super-resolution microscopy systems, including structured illumination microscopy and STED, with new DNA sequence design and imaging setting
  • As Exchange-Confocal requires sequential application of imager strands labeled with the same fluorophore, efficient imager strand removal is critical
  • The fluorescence intensity after washing with PBS decreased to the background level and was negligible compared to signal levels in the other images, confirming sufficiently efficient removal of imager strands from the solution
  • SV2 exists in both Outer Plexiform Layer and Inner Plexiform Layer, whereas Synapsin is only located in the IPL, similar to what has been reported in Salamander retina
  • Together with previous related Exchange-STED work applied to synthetic DNA nanostructures, our results show that DEI is generally compatible with SIM and STED microscopy and can be used for rapid multiplexed SR imaging
  • We have developed DNA-Exchange-Imaging as a rapid and versatile multiplexed imaging technique for both diffraction-limited and super-resolution in situ imaging in cells and in tissues

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