Role of the Protein Corona Derived from Human Plasma in Cellular Interactions between Nanoporous Human Serum Albumin Particles and Endothelial Cells

These findings reveal important information regarding the mechanisms used by vascular endothelial cells to internalize protein based-particulate materials exposed to blood plasma

Mikhail V. Zyuzin

2017

Scholarcy highlights

  • It is known that proteins from cell culture medium bind to the surfaces of particles, forming a so-called protein corona. In the literature, the formed protein interface is classified into a “hard” and “soft” corona, depending on the binding affinity and exchange rates of proteins to particles. Generally, it is considered that a hard corona consists of proteins that adhere to the particle surface strongly enough to survive interactions with the cell membrane and are internalized with particles together upon endocytosis
  • A t-test showed that the different dwelling times are statistically significant, see the Supporting Information § 2.2. These data suggest that the protein corona formed from incubation in human plasma plays a role on how long particles colocalize with early endosomes
  • Up to incubation times of 6 h, human serum albumin particles are internalized by HUVECs to a higher amount than HSA-human plasma particles, as indicated by the temporal increase of yellow fluorescence originating from particles in acidic intracellular vesicles
  • The current study shows that a protein corona forms on pure protein particles
  • The presence of additional proteins derived from human plasma can slow down particle binding to the extracellular membrane, increase the dwelling time in early endosomes, and reduce the amount of internalized particles within the first hours of cell exposure to particles
  • It has been shown that before bringing such particle systems into a clinical use, it is important to perform preliminary tests dedicated to the influence of the environment in vivo on particle behavior
  • This should aid the prediction of possible effects of such materials in vitro and in vivo

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