Cytochrome c Mutants for Superoxide Biosensors

We found that the E66K mutant-based electrode has a clearly higher sensitivity in comparison with the wild-type-based sensor while retaining the high selectivity and showing a good storage stability

Franziska Wegerich

2009

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  • Most electronic Supporting Information files are available without a subscription to ACS Web Editions
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  • The mutated forms of this electron-transfer protein are used as sensorial recognition elements for the amperometric detection of the reactive oxygen radical
  • The 11 mutants of human cyt c expressed in the course of this research have been characterized by UV−vis spectroscopy, circular dichroism, and NMR spectroscopy to verify overall structure integrity as well as axial coordination of the heme iron
  • Four mutants show a higher reaction rate with the radical as compared to the wild type. These mutants are used for the construction of superoxide sensors based on thiol-modified gold electrodes and covalently fixed proteins
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