Carbon Dots for Multiphoton Bioimaging

We found and reported11 that nanosized pure carbon particles may be surfacepassivated to exhibit bright photoluminescence in the visible

Li Cao; Xin Wang; Mohammed J. Meziani; Fushen Lu; Haifang Wang; Pengju G. Luo; Yi Lin; Barbara A. Harruff; L. Monica Veca; Davoy Murray; Su-Yuan Xie; Ya-Ping Sun


Scholarcy highlights

  • Two-photon fluorescence materials have attracted much recent attention for their many promising applications, especially in the growing field of biomedical imaging. Among the best performing two-photon fluorescence materials are semiconductor quantum dots such as CdSe and related core—shell nanoparticles. These quantum dots have been demonstrated in various optical imaging experiments in vitro and in vivo. At the same time, heavy metals as the essential elements in available high-performance semiconductor quantum dots have prompted serious health and environmental concerns in the community and beyond
  • The specimen for optical microscopy was prepared by first dropping a small aliquot of the aqueous solution on cover glass and evaporating the water
  • A Leica confocal fluorescence microscope equipped with an argon ion laser and a femtosecond pulsed Ti:sapphire laser was used
  • In an exploratory experiment to demonstrate the potential of C-Dots for cell imaging with twophoton luminescence microscopy, human breast cancer MCF-7 cells were cultured in terms of the established protocol
  • Upon incubation with the C-Dots in an aqueous buffer at 37 °C, the MCF-7 cells became brightly illuminated when imaged on the fluorescence microscope with excitation by 800 nm laser pulses
  • Available results from exploratory experiments of luminescence imaging in vitro suggest that the C-Dots are internalized into the human breast cancer cells likely through endocytosis, demonstrating the potential of the C-Dots in cell imaging with two-photon luminescence microscopy

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