Versatile Photosensitizers for Photodynamic Therapy at Infrared Excitation

The new design of versatile PDT photosensitizers we propose is schematically shown in Figure 1

Peng Zhang; Wim Steelant; Manoj Kumar; Matthew Scholfield

2007

Scholarcy highlights

  • A new type of photosensitizers used in photodynamic therapy, which is based on photon upconverting nanoparticles, is reported
  • It has been shown in previous reports that the intensity decrease of anthracenedipropionic acid emission follows an exponential decay over time, as ADPA being quenched by the generated singlet oxygen. 1516 The data points in Figure 3 could be nicely fit into an exponential decay function, with a correlation coefficient of 0.9992
  • The major difference is that the Merocyanine 540-coated PUNPs were excited by IR irradiation, while those others were excited by visible irradiation
  • A mouse monoclonal antibody, anti-MUC1/episialin, which is highly specific towards the MCF-7/AZ cells, was covalently attached to the M-540-coated PUNPs, following similar procedures described previously
  • MCF-7/AZ cells in suspension were incubated with propidium iodide, a fluorescence dye that stains the nuclei red once the cell is dead or dying, and the antibodyfunctionalized M-540-coated PUNPs
  • Control experiments with IR irradiation but without nanoparticles had been done, which showed that IR irradiation alone did not cause cell death. These results demonstrate that photosensitizers of the proposed design have great potential to become the generation PDT drugs
  • 18 in vivo PDT studies using these newly-designed photosensitizers are planned to be carried out along with the optimization of parameters, such as the type and amount of photosensitizing molecules loaded in the silica layer, the amount of antibody coated on the silica surface, and the intensity of the infrared irradiation

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