Effect of Surface Functionalization of MCM-41-Type Mesoporous Silica Nanoparticles on the Endocytosis by Human Cancer Cells

We report on the synthesis of a series of organically functionalized MSNs and investigate the mechanism and efficiency of endocytosis of these materials with different charge profiles on human cervical cancer cells

Igor Slowing; Brian G. Trewyn; Victor S.-Y. Lin

2006

Scholarcy highlights

  • Recent studies on the interaction between surface-functionalized inorganic nanoparticles and animal cells have shown great potential for utilizing these size-defined nanomaterials for various biomedical and biotechnological applications, such as cell type recognition, disease diagnosis, intracellular imaging, and drug/gene delivery
  • An important prerequisite would be to understand the mechanism of cellular uptake of these surface-functionalized mesoporous silica nanoparticles, so that the uptake of MSNs by different cell types can be regulated
  • To the best of our knowledge, no report has examined the effect of surface functional groups on the cellular uptake properties of these mesoporous silicabased nanoparticles
  • We report on the synthesis of a series of organically functionalized MSNs and investigate the mechanism and efficiency of endocytosis of these materials with different charge profiles on human cervical cancer cells
  • The MCM-41-type mesoporous structure with a 2.4 nm average pore diameter and a surface area of 850 m2/g was confirmed by powder X-ray diffraction, nitrogen sorption isotherms, and scanning and transmission electron microscopy measurements
  • To investigate the possible mechanism of endocytosis of the different MSNs, we tested the cellular uptake of these materials in presence of specific inhibitors
  • Supporting Information Available: Syntheses and characterizations of MSNs, cell cultures, flow cytometry, confocal fluorescence microscopy, and immunochemistry experiments

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