Facile Hydrothermal Synthesis of Chlorella-Derived Environmentally Friendly Fluorescent Carbon Dots for Differentiation of Living and Dead Chlorella

We developed chlorella-based carbonized polymer dots by a hydrothermal method

Deming Dong


Scholarcy highlights

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  • The judgment of microalgae viability is a vital procedure in the process of microalgae culture and treatment, which plays an important role in bioremediation, bioindication, and pharmacology fields
  • We developed chlorella-based carbonized polymer dots by a hydrothermal method. Due to their small average size of 5.0 nm, obvious excitation-dependent emission, stable fluorescence properties, and low toxicity, c-CPDs could be used for distinguishing living or dead chlorella by testing different fluorescence characteristics of c-CPD-labeled chlorella
  • This work provided a method to prepare environmentally friendly carbon dots using microalgae, which had potential to be prepared on a large scale and might be applied feasibly in the preparation of doped CDs by controlling the growth of chlorella
  • Optimization of reaction conditions for the synthesis of CPDs; optical properties of b-CPDs; effect of different feeding ratios on CPDs properties; stability analysis of c-CPDs; fluorescence spectra for the comparison of living chlorella, living chlorella cocultured with c-CPDs, and c-CPDs; fluorescence decay curve of c-CPDs, as well as living or dead chlorella with and without the treatment of c-CPDs; and the table of their fluorescence lifetime
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