Solution Conformations of Peroxiredoxins Visualised by Volta Phase Plates

We have recently shown that Volta phase plates extend the capabilities of cryo-EM and allow time-efficient structure determination of small protein complexes such as Prx3 dodecamers at significantly higher resolution than has previously been achievable

Mazdak Radjainia; Maryam Khoshouei; Matthew Belousoff; Radostin Danev

2016

Scholarcy highlights

  • Single particle cryo-EM enables three-dimensional structure determination of protein complexes without the need for crystals
  • Peroxiredoxins are a large family of peroxidases that are found in all kingdoms of life and play a critical role in removal of reactive oxygen species, hydrogen peroxide
  • It has been shown that only dimer formation is required for H2O2 detoxification; with the exact structure/function correlates of rings and filaments remaining unclear
  • We have recently shown that Volta phase plates extend the capabilities of cryo-EM and allow time-efficient structure determination of small protein complexes such as Prx3 dodecamers at significantly higher resolution than has previously been achievable
  • Our representation of the Prx3 dodecamer suggests that conformational changes in the active sites are concomitant with movements at the inter-dimeric domains, thereby enabling cross-talk between dimers
  • Besides revealing structural information on the significance of Prx3 oligomeric states, this study sets the stage for routine use of VPP single particle analysis for dynamic structure determination as a highresolution technique that is complementary to X-ray crystallography and Small Angle X-ray Scattering

Need more features? Save interactive summary cards to your Scholarcy Library.