[14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent

This chapter discusses the analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent

Vernon L. Singleton; Rudolf Orthofer; Rosa M. Lamuela-Raventós

2004

Scholarcy highlights

  • This chapter discusses the analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent
  • The assay is inclusive of monophenols and gives predictable reactions with the types of phenols found in nature
  • Judicious use of the assay—with consideration of potential interferences in particular samples and prior study if necessary—can lead to very informative results. Aggregate analysis of this type is an important supplement to and often more informative than reems of data difficult to summarize from various techniques, such as high-performance liquid chromatography that separate a large number of individual compounds .The predictable reaction of components in a mixture makes it possible to determine a single reactant by other means and to calculate its contribution to the total FC phenol content
  • Relative insensitivity of the FC analysis to many adsorbents and precipitants makes differential assay—before and after several different treatments—informative

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