The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

The results show that the method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins

K Weber

2021

Scholarcy highlights

  • Forty proteins with polypeptide chains of well characterized molecular weights have been studied by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate following the procedure of Shapiro, Vifiuela, and Maize1
  • When the electrophoretic mobilities were plotted against the logarithm of the known polypeptide chain molecular weights, a smooth curve was obtained
  • Two procedures were used to examine the relationship between electrophoretic mobilities and molecular weights of various proteins
  • The molecular weights for the different polypeptide chains are taken from Table I, in which the proteins we studied by SDS gel electrophoresis are listed
  • The binding of dodecyl sulfate ions to proteins has been shown for several protein moleculesolyacrylamide
  • How reproducible are the results obtained by SDS gel electrophoresis and how reliable are the molecular weights obtained? The results presented above quite clearly show that a high degree of reproducibility in the determination of the electrophorctic mobility can be obtained whether the proteins are run on different gels or on the same gel
  • The results show that the method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins

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