Biological disulfides: the third messenger? Modulation of phosphofructokinase activity by thiol/disulfide exchange.

GSH at pH 8.0 is 7.1 in the absence of substrates and 2+eq.5uCiinoliAbtSrhiSeuGmp+rceoGsneSsnHtcaewonfta0fso.1fromuth~nedArteToaPbc.teioF3no.1rCactooApmHSHp8a.0+r.isTGohnS,eSsGtehe regulation by thiol/disulfide exchange when we found that hydroxymethylglutaryl-CoenzymeA reductase is rapidly and reversibly inactivated by disulfides in general, and by Coenzyme A disulfide in particular

H F Gilbert

2021

Scholarcy highlights

  • Tivated atpH 8.0 by incubation witlhow concentrations there havebeen a growing number of reports of the of oxidized glutathione, Coenzyme A glutathione mixeedffects of thiol/dis&ide exchange on enzyme activity, this disulfide, and oxidized CoenzymAe
  • GSH at pH 8.0 is 7.1 in the absence of substrates and 2+eq.5uCiinoliAbtSrhiSeuGmp+rceoGsneSsnHtcaewonfta0fso.1fromuth~nedArteToaPbc.teioF3no.1rCactooApmHSHp8a.0+r.isTGohnS,eSsGtehe regulation by thiol/disulfide exchange when we found that hydroxymethylglutaryl-CoenzymeA reductase is rapidly and reversibly inactivated by disulfides in general, and by Coenzyme A disulfide in particular
  • Since reduced phosphofructokinase has multraces of oxygen in the system is shown by the fact thavtalues tiple binding sites of differing affinities and the binding conof enzyme activity and the/ ratios were the stant for interaction of ATP with the oxidized enzyme is not same at 16 and 24 4 of incubation
  • These observations suggest that mechanism of Equation7requires an' term in the phosphofructokinase contains one or more reactive sulfhydryl equilibrium expression for the reaction, computer simulation groups that areessential for catalytic showed that the dataof Fig. 4 could be adequately described activity
  • That the inacti- phosphofructokinase inactivation by biological disulfides is vation is not inhibited by AMP, CAMP, or citrate requires currently under investigation
  • ThioL/DisuLfideExchange ofPhosphofructokinase regulation of enzyme activity by the process of thiol/disulfide Whereas, we have shown thatphosphofructokinase is reversexchange, and that the ratio/ could serve asa ibly inactivated by oxidation, Nakashamaet al. and biological third messenger
  • Thiol/DisulfideExchange of Phosphofructokinase metabolic regulation by thiol/disulfide exchange, direct experimental support for the coupling of enzyme activity to the thiol/disulfide ratio in uiuo will be required

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