Serum and monoclonal antibodies against the chick intestinal receptor for 1,25-dihydroxyvitamin D3. Generation by a preparation enriched in a 64,000-dalton protein.

Munication, we report the partial purification of the chick intestinal receptor for 1,25(OH)~D3, anidts use as antigen in the immunization of two male Lewis rats

J W Pike

2021

Scholarcy highlights

  • Polyclonal serum antibodies to the 1,25-dihydroxy- Vitamin D3 undergoes a series of hydroxylations in liver vitamin D3 receptor have been obtained and kidney to 1,25-dihydroxyvitamin Ds, the active form of through hyperimmunization of two Lewis rats with the parent compound
  • The re- cated in a host of biological responses ranging from its tradiceptor protein was isolated from 10 kg of chick intestine via Polymin P fractionation, DNA and blue dextran affinity techniques, and gel filtration, and its presence in the preparation tentatively ascribed to a predominant species of 64,000 daltons on a sodium dodecyl sulfate-polyacrylamide gel
  • Whether the receptor mediates this cells from both Lewis rats were fused with the nonse- action on RNA synthesis has yet to be determined
  • Bation of radiolabeled 1,25(OH)zD3receptor with hybrideffects such as stimulation of protein synthesis and/or secremedia followed by second antibody immunoprecipitation, resulted in the identification of four hybridomas secreting monoclonal antibodies to the1,25(OH)zD3receptor
  • Munication, we report the partial purification of the chick intestinal receptor for 1,25(OH)~D3, anidts use as antigen in the immunization of two male Lewis rats
  • Significant titers of anti-1,25(OH)zD3receptor antibodies can was most useful inproviding a effective and be raised by repeated immunization of Lewis rats witha sensitive screeningtechnique for evaluating anextensive numpartially purified chick intestinal receptor preparation

Need more features? Save interactive summary cards to your Scholarcy Library.