Prediction, mapping and validation of tick glutathione S-transferase B-cell epitopes

This study revealed that the predicted conserved B-cell epitopes within the five tick GST sequences were localized on the surface of the respective GST homodimers

Charles Ndawula

2020

Scholarcy highlights

  • In search of ways to address the increasing incidence of global acaricide resistance, tick control through vaccination is regarded as a sustainable alternative approach
  • The conserved epitopes predicted within the sequences were mapped on the homodimers of the respective tick GSTs, and the corresponding peptides were independently used for rabbit immunization experiments
  • Based on the dot blot assay, the immunogenicity of the peptides and their potential to be recognized by corresponding recombinant glutathione S-transferase anti-sera raised by rabbit immunization in a previous work were investigated
  • This study revealed that the predicted conserved B-cell epitopes within the five tick GST sequences were localized on the surface of the respective GST homodimers
  • Given that a few proteasomal cleavage sites were found within the conserved epitope sequences of the four GSTs, the sequences could contain a T-cell epitope
  • These data support the claim that the rGSTs, used in the previous study, contain conserved B-cell epitopes, which elucidates why the rGST anti-sera cross-reacted to non-homologous tick GSTs
  • These data support the claim that the recombinant glutathione S-transferase, used in the previous study, contain conserved B-cell epitopes, which elucidates why the rGST anti-sera cross-reacted to non-homologous tick GSTs. Taken together, the data suggest that the B-cell epitopes predicted in this study could be useful for constituting epitope-based GST tick vaccines

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