Freeze-dried snake antivenoms formulated with sorbitol, sucrose or mannitol: Comparison of their stability in an accelerated test

These results suggest that sucrose is a better stabilizer than mannitol and sorbitol in the formulation of freeze-dried antivenoms under the studied conditions

MarĂ­a Herrera

2014

Scholarcy highlights

  • Freeze-drying or lyophilization is the most commonly used method for preparing solid proteins which are physically or chemically unstable in aqueous solution
  • This study investigated the use of sucrose, mannitol and sorbitol as stabilizers for snake antivenoms formulated as freeze-dried products
  • After six months of storage at 40 C, all the formulations presented the same residual humidity content, but significant differences were observed in turbidity, reconstitution time and electrophoretic pattern
  • All formulations, except antivenoms freeze-dried with mannitol, exhibited the same potency for the neutralization of lethal effect of B. asper venom
  • The 5% sucrose formulation showed the best stability among the samples tested, while the mannitol and sorbitol formulations underwent significant destabilization and turned brown
  • Results indicate that sucrose could perform as a better stabilizer than mannitol and sorbitol in the formulation of freeze-dried antivenoms
  • This formulation may become a good alternative for the production of more stable antivenoms in regions of the world where high temperatures are common and the cold chain is poor

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