Toxicological assessment of multi-walled carbon nanotubes on A549 human lung epithelial cells

Cell uptake was analysed by MWCNT-COOH, FITC labelled and the effect of internalisation was evaluated on the endocytic apparatus, mitochondrial compartment and DNA integrity

Giuseppa Visalli; Maria Paola Bertuccio; Daniela Iannazzo; Anna Piperno; Alessandro Pistone; Angela Di Pietro

2014

Scholarcy highlights

  • Cell uptake was analysed by MWCNT-COOH, FITC labelled and the effect of internalisation was evaluated on the endocytic apparatus, mitochondrial compartment and DNA integrity
  • We observed a correlation between MWCNTs uptake and lysosomal dysfunction and an inverse relationship between these two parameters and cell viability
  • Pristine-MWCNT caused a time- and dose-dependent ROS increase and higher levels of lipid hydroperoxides compared to the controls
  • Based on the physical and chemical features of MWCNTs, several toxicological pathways could be activated in respiratory epithelium upon their inhalation
  • Lysosomal dysfunction was able to trigger further toxic effects

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