An ultrasensitive homogeneous aptasensor for carcinoembryonic antigen based on upconversion fluorescence resonance energy transfer

We reported an ultrasensitive homogeneous aptasensor based on fluorescence resonance energy transfer between upconversion nanoparticles and graphene oxide for Carcinoembryonic antigen detection

Yujie Wang; Zikai Wei; Xianda Luo; Quan Wan; Rongliang Qiu; Shizhong Wang


Scholarcy highlights

  • Carcinoembryonic antigen has been recognized as one of the most important tumor markers
  • Excellent performance is obtained in both buffer solution and human serum samples
  • The results were correlated to those obtained from commercial chemiluminescence kits
  • The CEA aptamer modified upconversion nanoparticles can bind to the surface of graphene oxide through π-π stacking interaction, resulting in fluorescence quenching due to the energy transfer from UCNPs to GO
  • After the introduction of CEA, the CEA aptamer preferentially combined with CEA to form three-dimensional structure which made UCNPs-aptamer dissociate from the GO, blocking the energy transfer process
  • The aptasensor could monitor CEA level directly in human serum and the results were strongly correlated with commercial chemiluminescence kits
  • The excellent detection performance suggested promising prospect of the aptasensor in practical application

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