Morphological, transcriptomics and phytohormone analysis shed light on the development of a novel dwarf mutant of cabbage (Brassica oleracea)

Expression of key genes related to indole-3-acetic acid, BR and GA were down and those related to salicylic acid, jasmonic acid, abscisic acid and ET were up

Miaomiao Xing; Henan Su; Xing Liu; Limei Yang; Yangyong Zhang; Yong Wang; Zhiyuan Fang; Honghao Lv

2019

Scholarcy highlights

  • Expression of key genes related to indole-3-acetic acid, BR and GA were down and those related to salicylic acid, jasmonic acid, abscisic acid and ET were up
  • Change in GA4, JA, ET, SA and ABA measured by LC–MS/MS were consistent with the transcriptome results
  • We first discovered a spontaneous cabbage dwarf mutant 99–198dw, which exhibits a dwarf stature, wrinkled leaves, non-heading, and substantially reduced self-fertility compared with the wild-type 99–198; the underlying molecular mechanism of its dwarfism is unknown
  • Key genes in stress-resistant pathways were mostly upregulated, including salicylic acid, jasmonic acid, abscisic acid, ethylene, etc., while the differentially expressed genes reported to be related to plant height, such as those involved in the gibberellin, brassinolide, indole-3-acetic acid, and strigolactone pathways were mostly downregulated
  • It was speculated that mutation of certain DEG related to cell division or participating in signalling pathway of phytohormones like GA, BR, IAA, and SL were the cause of dwarf
  • These results are informative for the elucidation of the underlying regulatory network in 99–198dw and enrich our understanding of plant dwarf traits at the molecular level

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