Chemical identification of 18-hydroxycarlactonoic acid as an LjMAX1 product and in planta conversion of its methyl ester to canonical and non-canonical strigolactones in Lotus japonicus

These results demonstrated that LjMAX1 is the crucial enzyme in the biosynthesis of Lotus SLs

Narumi Mori; Aika Sado; Xiaonan Xie; Kaori Yoneyama; Kei Asami; Yoshiya Seto; Takahito Nomura; Shinjiro Yamaguchi; Koichi Yoneyama; Kohki Akiyama

2020

Scholarcy highlights

  • Strigolactones are a group of apocarotenoid compounds identified as germination stimulants of root parasitic plants about 50 years ago
  • To confirm the involvement of Lotus japonicus MORE AXILLARY GROWTH 1 homolog in the biosynthesis of SLs, one LORE1 insertion mutants were obtained from the Lotus Base mutant collection
  • We analyzed the SL content in both WT and the Ljmax1 mutant. We grew both genotypes in hydroponic solution without Pi to induce SL production. 5DS and LL were detected in the root exudates of WT, but were not detected in those of the Ljmax1 mutant
  • These results demonstrated that LjMAX1 is the crucial enzyme in the biosynthesis of Lotus SLs
  • To investigate whether the LjMAX1 product carlactonoic acid is a biosynthetic precursor for SLs in planta, we examined the conversion from exogenous CLA into SLs using L. japonicus WT
  • We showed that LjMAX1 is the crucial enzyme in the biosynthesis of
  • To a solution of 13C-18-TBSO-methyl carlactonoate in THF was added at room temperature acetic acid and water, and the mixture was stirred for 3 h

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