Proteomic and activity profiles of ascorbate–glutathione cycle enzymes in germinating barley embryo

Enzymes involved in redox control are important during seed germination and seedling growth

Birgit C. Bønsager

2010

Scholarcy highlights

  • Enzymes involved in redox control are important during seed germination and seedling growth
  • Dehydroascorbate reductase activity was present at 4 h post imbibition and first decreased by 9-fold until 72 h PI followed by a 5-fold increase at 144 h PI
  • While gel spots containing ascorbate peroxidase showed intensity changes consistent with the activity profile from 0 to 72 h PI, dehydroascorbate reductase spot intensities indicated that post-translational regulation may be responsible for the observed changes in activity
  • Transcript profiling, 2D-western blotting and mass spectrometric characterization of multiple APX spots demonstrated the presence of APX1 and minor amounts of APX2

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