E-cadherin is a receptor for the common protein pneumococcal surface adhesin A (PsaA) of Streptococcus pneumoniae

Using recombinant pneumococcal surface adhesin A covalently bound to fluorescent spheres, we show PsaA binds to NP cells through interaction with the human cellular receptor, E-cadherin

Julie M. Anderton; Gowrisankar Rajam; Sandra Romero-Steiner; Susan Summer; Andrew P. Kowalczyk; George M. Carlone; Jacquelyn S. Sampson; Edwin W. Ades

2007

Scholarcy highlights

  • Streptococcus pneumoniae binds to nasopharyngeal epithelial cells in the first steps of nasopharyngeal carriage and colonization through bacterial adhesins
  • Identification of a receptor for pneumococcal surface adhesin A on human epithelium will aid in understanding the pathogenesis of this bacterium
  • Using recombinant PsaA covalently bound to fluorescent spheres, we show PsaA binds to NP cells through interaction with the human cellular receptor, E-cadherin
  • SDS-PAGE silver stain analysis demonstrates binding of PsaA to E-cadherin
  • If NP cells were preincubated with anti-E-cadherin antibody, there was a significant decrease in PsaA-coated fluosphere adherence to the cells
  • This work identifies E-cadherin as a receptor on human epithelial cells for the pneumococcal surface adhesin, pneumococcal surface adhesin A

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