Effect of cryoprotection on particle size stability and preservation of chitosan nanoparticles with and without hyaluronate or alginate coating

Due to exceptional unique physical and biological characteristics, the polysaccharides have got prime attention as a carrier in drug delivery systems

Abdulaziz Almalik

2017

Scholarcy highlights

  • Due to exceptional unique physical and biological characteristics, the polysaccharides have got prime attention as a carrier in drug delivery systems
  • The ionic gelation method for the preparation of chitosan nanoparticles as well as the hyaluronic acid and ALG coated CS-NPs was found to be an effective technique which was confirmed by the Dynamic Light Scattering measurements for particle size distribution, zeta potential measurements and estimation of polydispersity index of the prepared NPs
  • Zeta potentials were recorded for the three different types of the NPs directly after synthesis and conversion of surface charge of NPs was found apparent which can be seen in the zeta potential curves where the positive zeta potential value of CS-NPs changed to negative values when CS-NPs were coated with HA and ALG
  • The average size of CS-NPs was found around nm, while coating of these CS-NPs with HA increased an average particle size around 100 nm and shifting of zeta potential value from positive to negative was found, which was due to the polyanionic nature of the HA molecules
  • A great shifting of zeta potential value from a positive to a high negative was found. These apparent changes in the zeta potential values were expected, because primarily, ALG has high negative charge density, secondly, ALG could be adsorbed rapidly and efficiently on the highly cationic surfaces of CS-NPs and resulted in a significantly higher negative surface charge density on the coated NPs which was the main cause of the significant increase in the size of
  • An obvious change in the mean particle size was observed after lyophilization of the coated/ uncoated CS-NPs, but the application of cryoprotectants provided a significant reduction in the particle size after lyophilization and prevented aggregation among the NPs
  • The physical stability results suggested that the rate of changes of uncoated particle size was significantly greater and faster when it was compared with hyaluronic acid and ALG-coated chitosan nanoparticles, which indicated the involvement of aggregates and cluster formation of uncoated CSNPs and caused the higher particle size enhancement during storage

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