Revealing fibrinogen monolayer conformations at different pHs: Electrokinetic and colloid deposition studies

Adsorption mechanism of human fibrinogen on mica at different pHs is studied using the streaming potential and colloid deposition measurements

MaƂgorzata Nattich-Rak

2014

Scholarcy highlights

  • Adsorption mechanism of human fibrinogen on mica at different pHs is studied using the streaming potential and colloid deposition measurements
  • Electrokinetic properties of fibrinogen monolayers on mica are characterized
  • The fibrinogen monolayers are produced by a controlled adsorption under diffusion transport at pH of 3.5 and 7.4
  • The orientation of molecules and monolayer structure is evaluated by the colloid deposition measurements involving negatively charged polystyrene latex microspheres, 820 nm in diameter
  • At pH 7.4 this critical distance is 23 nm. This confirms that fibrinogen monolayers formed at both pHs are characterized by the presence of the side-on and end-on oriented molecules that prevail at higher coverage range
  • It is concluded that the colloid deposition method is an efficient tool for revealing protein adsorption mechanisms at solid/electrolyte interfaces

Need more features? Save interactive summary cards to your Scholarcy Library.