Macrophage functionality and homeostasis in response to oligoethyleneglycol-coated IONPs: Impact of a dendritic architecture

In the present study we investigated the biocompatibility of iron oxide nanoparticles grafted with linear or generation 1 or 2 dendronized oligoethyleneglycol units in THP-1-derived macrophages

Anne Casset

2018

Scholarcy highlights

  • The iron oxide nanoparticles exhibit useful properties such as magnetic behaviour and have been developed for various applications in technology and medicine
  • This study aimed to evaluate the biocompatibility of three IONPs dysplaying a biphosphonate coating grafted with different oligoethyleneglycol-based dendrons on THP-1-derived macrophages
  • Our results showed that the IONPs coated with a linear molecule, namely NP10COP@2P, displayed cytotoxic effects upon short-term exposure, whereas the two other IONPs coated with generation 1 and 2 dendrons, namely NP10COP@2PG1 and NP10COP@2PG2, exhibited better biocompatibility
  • The toxic responses evoked by NP10COP@2P included release of the pro-inflammatory cytokine IL-8, oxidative stress and NLRP3 inflammasome activation, and were related to higher cellular uptake of the NPs
  • These in vitro toxicological assessment seem to make the IONPs grafted with generation 1 or 2 dendronized oligoethyleneglycol units good candidates for preclinical in vivo ADME-T evaluations
  • Due to the great diversity in surface coatings, a wide range of concentrations, from a few μg/mL to 1 mg/mL, was tested and non-toxic concentrations were determined. These non-toxic concentrations appeared as coatingspecific, reaching 150 μg Fe/mL for carboxydextran-coated IONPs, and 200 μg Fe/mL for citrate-coated IONPs and dextran-coated IONPs
  • Efficient internalization of silica-coated iron oxide nanoparticles of different sizes by primary human macrophages and dendritic cells

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