A simple improved desolvation method for the rapid preparation of albumin nanoparticles

Preparation procedure was simplified by using an apparatus for the addition of ethanol

Ali Jahanban-Esfahlan

2016

Key concepts

Scholarcy highlights

  • Preparation procedure was simplified by using an apparatus for the addition of ethanol
  • The Procedure used for the nanoparticles preparation was simplified by using a designed apparatus for controlling the addition of ethanol and it was used instead of conventional tubing pump which enabled the preparation of nanoparticles under defined conditions
  • Nanoparticles with smaller size were obtained under experimental conditions without the presence of salts or the use of buffers, 250 mg of protein/4 ml water, 5 mg cross-linker, the addition of 4 and 8 ml ethanol by using the designed apparatus to the HSA and BSA solution, respectively
  • By using this improved method, BSA and HSA nanoparticles of the size around 100 nm and polydispersity below 0.2 were obtained

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