Fish cell cultures as in vitro models of pro-inflammatory responses elicited by immunostimulants

The responses varied depending upon the concentration of the immunostimulants and the particular cell line, our results demonstrate that those activities were enhanced in the TSS and TPS-2 cell lines after exposure to any of the immunostimulants

C. Fierro-Castro; L. Barrioluengo; P. López-Fierro; B.E. Razquin; B. Carracedo; A.J. Villena

2012

Scholarcy highlights

  • We have tested the elicitation of innate defence-related responses in two stromal cell lines derived from the spleen and the pronephros of rainbow trout after they were exposed to different concentrations of lipopolysaccharide, levamisole, or polyinosinic polycytidylic acid
  • The responses varied depending upon the concentration of the immunostimulants and the particular cell line, our results demonstrate that those activities were enhanced in the TSS and TPS-2 cell lines after exposure to any of the immunostimulants
  • These results indicate that the stromal cells of the main lympho-haemopoietic organs of O. mykiss develop innate defence responses, which are enhanced by well-known immunostimulants
  • Such enhancement of the defence responses in the TSS and TPS-2 cell lines could be elicited when they were exposed to conditioned supernatants from levamisole- or poly I:C-stimulated HK macrophage cultures, demonstrating that the haemopoietic stromal cells respond to macrophage-derived factors
  • The results are discussed considering the role of the lympho-haemopoietic stromal cells in the innate immune responses, and the possibility of using histiotypic cell cultures of non-leucocyte cells of the haemopoietic organs to develop in vitro methods to select new immunostimulant candidates for aquaculture

Need more features? Save interactive summary cards to your Scholarcy Library.