Biochemical identification of a hydroperoxide derivative of the free 8-oxo-7,8-dihydroguanine base

By using biochemical and cell biological assays, we showed that in the presence of molecular oxygen, the free 8-oxoG base transformed to a highly reactive hydroperoxide

Gyorgy Hajas; Attila Bacsi; Leopoldo Aguilerra-Aguirre; Peter German; Zsolt Radak; Sanjiv Sur; Tapas K. Hazra; Istvan Boldogh

2011

Scholarcy highlights

  • Reactive oxygen and other oxidizing species are the most common chemical entities to modify constituents of DNA, RNA and the nucleotide pool
  • We studied whether the free 8-oxoG base could function in a manner similar to that of its intrahelical nucleoside form
  • The human myelomonocytic KG-1 cells were grown in Iscove's Modified Dulbecco's Medium
  • Further oxidation of 8-oxoG is well established, and its oxidized derivatives are well-characterized in the context of DNA and nucleosides
  • It has been shown that purine ribonucleotides are scavengers of radicals including OH, and that free 8-oxodeoxyguanosine provides the strongest protection against oxidation of DNA damage
  • By utilizing the Amplex Red assay, the hydroperoxide generated in 8-oxoG solution was a stronger oxidant than organic peroxides, e.g., cumene hydroperoxide or tert butyl hydroperoxide
  • KG-1 cells allowed us to show that, parallel with the release of a free 8-oxoG base, there was an increase in DCF fluorescence

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